VIII SIMPOSIO INTERNAZIONALE ICPBR

HAZARDS OF PESTICIDES TO BEES

Bologna, 4-6 Settembre 2002

Metodologie dei test e studi sugli effetti dei pesticidi sulle api

Intoxication of honeybees on chemical treated winter rape: problem of its verification

František Kamler (1), Dalibor Titera (1), Jirina Piškulová (1), Jana Hajšlová (2) and Katerina Maštovská (2)

(1) Bee Research Institute, Dol, CZ 252 66, Libcice nad Vltavou, Czech Republic. E-mail: titera@beedol.cz
(2) Institute of Chemical Technology, Department of food chemistry and analysis, Technická 5, CZ 160 00 Praha 6, Czech Republic

In the last 5 years were found in increased scope intoxication of bee colonies in connection with the treatment of winter rape against weevils in March and April. The treatment of the crop plant was based on the application of Regent 800 WG and Nurelle D.

Through the visual symptoms of die of bees was typical for intoxication, the chemical analyses of residues on bees and plants was negative.

The reported experiments were to clear the dynamics of the decomposition of chlorpyriphos and fipronil on plants and bees.

We took samples of plants and bees in various periods after the simulated rape treatment by preparations Nurelle and Regent. The plant and bee samples were stored to 4 days in room temperature.

Matrix was extracted by acetonitril, determination of fipronil and their degradation products by MB45950 (photodegradation) and MB46136 (oxidation) and the confirmations of results were carried out by GC/MS method. The determination of chlorpyriphos was done by GC/NPD method.

The content of active substances in stored plant samples was not rapidly changed. The content of active substances in dead bees stored to 4 days at room temperature was rapidly lower, but remained useful because of the applied methods.

Intoxication of bees after application of Regent and Nurelle are demonstrable if samples arrive at the laboratory in 4 days after plant treatment.